Immortalized human podocyte cell line

WitrynaA conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc Nephrol. 2002; 13: 630-638. Crossref; PubMed; Google Scholar, 20. Saleem M.A. Ni L. Witherden I. et al. Co-localization of nephrin, podocin, and the actin cytoskeleton: evidence for a role in podocyte foot process formation. WitrynaImmortalized Human Kidney Podocytes are developed by immortalizing primary human healthy kidney podocytes with SV40 Large T antigen. These kidney podocytes were …

Cell culture of podocytes. Conditionally immortalized human podocytes ...

Witryna17 maj 2012 · Conditionally immortalized human podocyte cell lines have been developed by transfection using both the temperature-sensitive mutant U19tsA58 of … WitrynaTo investigate changes on the cellular level, we used an immortalized human podocyte cell line and generated a stable knockdown of Sphk2 (Sphk2-kd) by a lentiviral transduction method. crystal world vic https://nakytech.com

CIHP-1 - Wikidata

WitrynaATCC cell lines immortalized human podocytes ab8 13 m saleem Cell Lines Immortalized Human Podocytes Ab8 13 M Saleem, supplied by ATCC, used in … WitrynaA breakthrough in cell biology research. Human telomerase reverse transcriptase (hTERT)–immortalized primary cells represent a breakthrough in cell biology … WitrynaImmortalized Human Podocytes are podocytes isolated from urine samples from healthy donor, and had been transformation with the thermosensitive SV40 large T … crystal world \\u0026 prehistoric journeys

(PDF) A Conditionally Immortalized Human Podocyte Cell Line ...

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Immortalized human podocyte cell line

Generating Conditionally Immortalised Podocyte Cell Lines from …

WitrynaImmortalized Primary Cells. Innoprot offer Human and animal primary cells immortalized with SV40 antigen T and other immortalization techniques. All immortalized cell lines has been characterized for specific markers and other for functional assays. Showing 1–16 of 23 results. 1 2. Witryna30 sie 2009 · podocyte markers in cultured urinary cells. Further study of urinary podocytes offers an open window to an understanding of glomerular disease. …

Immortalized human podocyte cell line

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WitrynaExperimental approach: Expression of TRPV1-4 channels was investigated at protein (immunocytochemistry, Western blot) and mRNA (Q-PCR) level in a conditionally immortalized human podocyte cell line. Channel function was assessed by measuring intracellular Ca 2+ concentration using Flou-4 Ca 2+ -indicator dye and patch clamp … WitrynaThe Human Podocyte Cell Line (CIHP-1) is a primary cell line isolated from human kidney. Podocytes are highly specialised, terminally differentiated cells with a complex …

WitrynaATCC cell lines immortalized human podocytes ab8 13 m saleem Cell Lines Immortalized Human Podocytes Ab8 13 M Saleem, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WitrynaImmortalized Human Kidney Podocytes (IM-HKP) provided by Innoprot have been developed by immortalizing primary human healthy kidney podocytes with SV40 …

Witryna11 lis 2009 · Conditionally immortalized human podocytes were cultured for up to 24 h with 375–750 μM palmitate. Functional effects on glucose uptake and ceramide production were measured. ... Reiser J, et al. A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc … WitrynaG.J. Audesirk, in Comprehensive Toxicology, 2010 13.24.2.3.5 Immortal cell lines. Immortal cell lines are an alternative to primary cultures. Frequently used cell lines …

WitrynaOther investigators have immortalized mouse and human neural stem cells using retroviral transduction of the v-myc oncogene (Snyder, 1995; ... 2007) and his team …

WitrynaOur normal human primary cells retain in vivo physiologies and custom lots are available from a diverse donor pool. ATCC hTERT-immortalized primary cells also display … dynamics 365 table referenceWitrynaA seminal breakthrough came about a decade ago with the documentation of differentiation in culture of primary rat and human podocytes and the subsequent development of conditionally immortalized differentiated podocyte cell lines that allow deciphering the decisive steps of differentiation and function of ‘in vivo’ podocytes. … crystal world victoriaWitrynaA stable SPL-kd cell line of human podocytes was generated by the lentiviral shRNA transduction method and was characterized for reduced SPL mRNA and protein levels and increased S1P levels. crystal world tucsonWitryna3 sty 2024 · In this study, conditionally immortalized human podocytes were used, ... This podocyte cell line proliferates under permissive conditions at 33 °C. When cultivated at 37 °C, the SV40 T-antigen is inactivated for cell differentiation. The culture medium for human podocytes was RPMI 1640 Medium (Gibco, via Thermo Fisher … dynamics 365 system architectureImmortalised cell lines are widely used as a simple model for more complex biological systems, for example for the analysis of the biochemistry and cell biology of mammalian (including human) cells. The main advantage of using an immortal cell line for research is its immortality; the cells can be grown indefinitely in culture. This simplifies analysis of the biology of cells which may otherwise have a limited lifetime. crystal world water park haridwarWitryna14 lut 2015 · Mouse podocytes immortalised by transduction with a temperature sensitive large T antigen SV40 construct as previously described 45, 46, were used … crystal world water park chargesWitryna1 mar 2010 · Evidence suggests that loss of podocytes into urine contributes to development of glomerular diseases; shed podocytes are frequently viable and … dynamics 365 tables and fields