Glut4 translocation assay

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August undefined, 2024

WebLe régime d’éviction est à la base de la prise en charge de l’allergie alimentaire. Par le passé, le régime était rigoureux et strict. WebOct 29, 2010 · Existing GLUT4 translocation assays are based on time-consuming immunoassays and are hampered by assay variability and low sensitivity. We describe a real-time, visual, cell-based qualitative GLUT4 translocation assay using CHO-HIRc-myc-GLUT4eGFP cells that stably express myc- and eGFP-tagged GLUT4 in addition to …

Observing GLUT4 Translocation in Live L6 Cells Using Quantum Dots …

WebGlucose Uptake Assay. ... while glucose ingestion and fatty acid oxidation were induced. 24 A previous study found that the translocation of GLUT4 and GLUT8 in the atria cells of healthy mice and type-1 diabetic mice was suppressed by … WebGLUT4 (SLC2A4) is the insulin-responding glucose transporter, found predominantly in muscle cells and adipocytes (fat cells). After a meal, glucose that is absorbed from the digestive system and circulates in the blood now stimulates the release of insulin from the pancreas (Figure 4.10).This insulin is the signal for a rapid transfer of GLUT4 in muscle … topic sentence about ethos

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WebJan 21, 2024 · Then, the fluorescence intensity ratio of the cell periphery to the perinuclear region was used to estimate GLUT4 translocation. With this assay, thousands of cells were analyzed in parallel through automated microscopy and image analysis. Although the presented screening system allows for the analysis of many cells in real time, the ... WebMay 13, 2024 · GLUT4 is the primary glucose transporter in adipose and skeletal muscle tissues. Its cellular trafficking is regulated by insulin signaling. Failed or reduced plasma membrane localization of... WebMay 13, 2024 · At basal state, GLUT4 is primarily distributed on the membranes of the trans-Golgi network (TGN), endosomes and 50–70 nm tubulo-vesicular structures known as the GLUT4 storage vesicles (GSVs) 7,8. topic research proposal

Specialized sorting of GLUT4 and its recruitment to the …

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WebGlut4 translocation assays are usually performed in differentiated myocytes or adipocytes. The requirement for differentiation makes such assays difficult, particularly in high throughput screen (HTS) mode, since differentiation extends the assay time line and makes assays more variable from well to well and particularly from batch to batch of ... WebNov 7, 2010 · The translocation of GLUT4 from the cytoplasm to the plasma membrane can be visualized by immunocytochemistry, using fluorophore-conjugated GLUT4-specific antibodies. Here, we describe a... topic segmentationWebDec 8, 2024 · It is crucial to determine the regulation of GLUT4 translocation and redistribution to the plasma membrane. The HA-GLUT4-GFP dual-reporter construct has become an important tool in the assessment of GLUT4 recycling in cultured adipocytes and myocytes. Through the use of light microscopy, this reporter construct allows for … topic selection definition

"WebJan 1, 2024 · GLUT4 translocation assay was carried on CHO-HIRC-myc-GLUT4 eGFP cell line; and a parallel glucose uptake study was carried out in differentiated 3T3L1 adipose and HepG2 cells. Briefly, a day before the experiment, 5 × 10 3 cells were plated in each well of a 96 well plate. " - Glut4 translocation assay

Glut4 translocation assay

Insulin stimulated GLUT4 translocation – Size is not everything!

WebDec 21, 1999 · Insulin induced the translocation of both mycGlut4 and endogenous Glut4 from the intracellular pool to the PM (Fig. 1 B and C ), indicating that transfected mycGlut4 undergoes insulin-regulated … WebThe agonist assay can be used to identify compounds that modulate translocation of GLUT4 and thereby affect glucose uptake. Insulin is used as reference agonist. Furthermore, the assay can potentially be formatted to screen for compounds that enhance the translocation of GLUT4 in response to insulin (i.e. insulin sensitizers).

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WebJan 1, 2024 · The assay could collect real time data of GLUT4 translocation from thousand of cells/ sample and from many such samples in one experiment. We validated the assay using Insulin, insulin... WebApr 20, 2024 · Figure 1 AMPK regulates GLUT4 translocation to the PM and glucose uptake in L6 myoblasts.(A) GLUT4 chimeric protein, Myc-GLUT4-mCherry, for the detection of GLUT4 translocation and insertion into the PM.(B, C) L6 myoblasts stably expressing Myc-GLUT4-mCherry were serum starved for 2 hours and treated with DMSO as control, …

WebTranslocation of the glucose transporter (GLUT4) to the cell surface in permeabilized 3T3-L1 adipocytes: effects of ATP insulin, and GTP gamma S and localization of GLUT4 to clathrin lattices. J Cell Biol 117: 1181–1196 Parton, R.G., Molero, J.C., Floetenmeyer, M., Green, K.M., James, D.E. 2002. WebJan 1, 2012 · Abstract. Insulin-stimulated glucose transporter 4 (GLUT4) translocation promoting glucose uptake is vital to glucose homeostasis and is a defined target of antidiabetic drug research. Existing functional assays to detect the process of GLUT4 translocation are hampered due to assay variability and low sensitivity, thus slowing …

WebApr 12, 2024 · transporter GLUT4 is kept intracellular and sorted dynamically, and GLUT4 translocation or insulin-regulated vesicular trafﬁc distributes it to the plasma membrane. One of the most WebMar 27, 2024 · GLUT4 translocation In skeletal muscle, translocation and redistribution of GLUT4 to the plasma membrane is a characteristic feature in increased glucose uptake and the utilization effect of insulin.

WebJan 1, 2012 · General GLUT4 translocation assays employ indirect methods such as western blot analysis of PM fractions, photoaffinity labeling, binding assay using cytochalasin B and qualitative assessment by immunofluorescence or immunoelectron microscopy ( Kozka et al., 1991, Smith et al., 1991 ).

WebFeb 11, 2009 · GLUT4 (glucose transporter 4) plays important roles in glucose homoeostasis in vivo. GLUT4 expression and function are diminished in diabetic human and animal subjects. The goal of the present study is to develop a cell-based assay for identifying negative regulators of GLUT4 translocation as potential targets for the … topic sentence discussing magnetic generatorsWebType 2 diabetes (T2D) is a chronic metabolic disease, which could affect the daily life of patients and increase their risk of developing other diseases. Synthetic anti-diabetic drugs usually show severe side effects. In the last few decades, plant-derived drugs have been intensively studied, particularly because of a rapid development of the instruments used … topic sentence articleWebAug 1, 2024 · Assessment of GLUT4 translocation in muscle and muscle cell lines has revealed insulin-stimulated increases at the cell surface of between two- and sixfold in human and rat muscles, respectively, in which the levels of cell surface GLUT4 were measured using affinity labelling approaches [29, 32, 33, 34 ]. topic sentence analytical essayWebGlut4 Translocation Assay/Surface Glut4 Labeling. Cells expressing HA-Glut4/GFP were incubated for 2 h in low serum medium (LSM; DMEM complete medium and 0.5% fetal bovine serum). Cells were then incubated with or without 100 n m insulin for 45 min. To label surface Glut4, cells were rapidly chilled on an ice-water slurry and labeled with LSM ... topic sentence and main ideaWebThe use of the GLUT4 translocation assay for the identification of new anti-diabetic drugs. L6 myotubes expressing hemagglutinin (HA) tagged GLUT4 were incubated with vehicle (Veh) or 10 mM... topic sentence for lord of the fliesWebAug 20, 2024 · The xanthene derivative, DS20060511, is a skeletal muscle cell-specific inducer of GLUT4 translocation. We screened our chemical library, composed of more than 100,000 compounds, using L6-GLUT4myc ... topic sentence definition elaWebOct 5, 2024 · Insulin stimulates glucose uptake in adipose tissue and skeletal muscle by inducing plasma membrane translocation of the glucose transporter GLUT4. Although the small GTPase Rac1 is a key regulator downstream of phosphoinositide 3-kinase (PI3K) and the protein kinase Akt2 in skeletal muscle, it remains unclear whether Rac1 also … topic sentence in a sentence